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nanodisc membrane scaffold protein spmsp1d1  (Addgene inc)


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    Structured Review

    Addgene inc nanodisc membrane scaffold protein spmsp1d1
    Nanodisc Membrane Scaffold Protein Spmsp1d1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nanodisc+membrane+scaffold+protein+spmsp1d1/pFLAG-4EHP(K134R)+(Plasmid+%2317348)/pmc12664993-170-5-13
    Average 93 stars, based on 12 article reviews
    nanodisc membrane scaffold protein spmsp1d1 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Calcium stabilizes the flexible N-terminal domain of the bacterial ion channel DeCLIC
    Article Snippet: .. A plasmid containing the circularized nanodisc membrane scaffold protein spMSP1D1 was obtained from Addgene (#173482). .. Purification of spMSP1D1 followed previously published protocols. ( ) For reconstitution in circularized nanodiscs, purified DeCLIC, spMSP1D1 and soybean polar lipid extract (Avanti) were mixed at a 1:2:40 M ratio, then incubated on ice for 1 h. Bio-Beads SM-2 resin (Bio-Rad) was added to the mixture, then gently rotated overnight at 4 °C.

    Article Title: Calcium stabilization of a flexible N-terminal domain in a pentameric ligand-gated ion channel
    Article Snippet: .. A plasmid containing the circularized nanodisc membrane scaffold protein spMSP1D1 was obtained from Addgene (#173482). ..

    Membrane:

    Article Title: Calcium stabilizes the flexible N-terminal domain of the bacterial ion channel DeCLIC
    Article Snippet: .. A plasmid containing the circularized nanodisc membrane scaffold protein spMSP1D1 was obtained from Addgene (#173482). .. Purification of spMSP1D1 followed previously published protocols. ( ) For reconstitution in circularized nanodiscs, purified DeCLIC, spMSP1D1 and soybean polar lipid extract (Avanti) were mixed at a 1:2:40 M ratio, then incubated on ice for 1 h. Bio-Beads SM-2 resin (Bio-Rad) was added to the mixture, then gently rotated overnight at 4 °C.

    Article Title: Calcium stabilization of a flexible N-terminal domain in a pentameric ligand-gated ion channel
    Article Snippet: .. A plasmid containing the circularized nanodisc membrane scaffold protein spMSP1D1 was obtained from Addgene (#173482). ..



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